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Optical Imaging Techniques in Cell Biology, by Guy Cox
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Since the word microscopy was coined in 1656, the evolution of the instrument has had a long and convoluted history. Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope’s technical progression happened in a series of fits and starts until the late 19th century. After Ernst Abbe perfected the “how” of lens design, achieving the theoretical limit imposed by wavelength, there came a revolution in subject matter or “what” could be studied by microscope.
Covering the entire field of microscopy, Optical Imaging Techniques in Cell Biology provides an overview of the technical evolution of the microscope and explains how the basics of optical microscopy led to the most advanced techniques employed today. The author addresses a vast array of topics including optical contrasting techniques, fluorescence, confocal versus widefield microscopes, lasers as a light source, and digital imaging, as well as the correction of aberrations that might arise. Building on this foundation, he then examines more advanced techniques such as quantitative fluorescence, fluorescence resonant energy, three-dimensional imaging, high-speed confocal microscopy, non-linear microscopy, and stimulated emission depletion.
Delivering a truly comprehensive work encompassing the scope and breadth of the field, the author brings a new level of understanding to the student, technician, researcher, or investigator working in the fascinating realm of optical microscopy.
- Sales Rank: #3912420 in eBooks
- Published on: 2006-11-06
- Released on: 2006-11-06
- Format: Kindle eBook
Most helpful customer reviews
0 of 0 people found the following review helpful.
Great Biological Imaging Overview
By D. Blustein
This is a very nicely written overview of biological imaging techniques. Explanations on a few of the topics are a bit overly technical but all in all, the writing is straightforward and clear. A good option for biologists in college and beyond.
0 of 0 people found the following review helpful.
Optical Imaging Techniques in Cell Biology (by Guy Cox)
By Christian T. K.-H. Stadtlander, Ph.D.
Cell biology ― a branch of biology in which researchers explore the various structures and functions of the cell ― is unimaginable without the use of imaging techniques. In the Introduction of his book, Guy Cox briefly reviews the historical events that led to the development of simple and compound microscopes as well as to sophisticated imaging techniques such as fluorescence-, phase contrast-, confocal-, and multiphoton microscopy. He also mentions issues in optical imaging that arose with spherical aberration, resolution, diffraction, and the theoretical limit imposed by the wavelength of light.
In the following chapter (Chapter 1), Cox provides in-depth information about the theory behind, the components of, and the practice of using the light microscope. He then turns his attention to optical contrasting techniques such as phase- and differential-interference-contrast (Chapter 2), fluorescence microscopic methods (Chapter 3), and issues in image capturing such as choice of film, camera, and filter (Chapter 4).
The next three chapters are devoted to confocal microscopy, including the use of various lasers as the light source (Chapter 5), the digital image acquisition methodology (Chapter 6), and the problems an investigator can encounter with aberration (Chapter 7). The eighth chapter is about nonlinear microscopy, using the example of multiphoton microscopy. In Chapter 9, he talks about the advantages of using high-speed confocal microscopy in cell biology. This is followed by a chapter about the deconvolution and image processing in confocal microscopy.
In Chapter 11, Cox describes three-dimensional (3D) imaging techniques. He wrote: “We believe that we see the world in three dimensions. This is not really so ― rather, we interpret the world in three dimensions.” He subsequently describes stereoscopy and the 3-D reconstruction of the image. Green fluorescent protein (GFP) is the topic of Chapter 12. Cox points out that this quite stable protein (it can survive relatively harsh treatment conditions in the laboratory) has revolutionized cell biology in that it can be used as “an expressible marker that is directly visible in the fluorescence microscope.” In Chapter 13, he discusses fluorescent staining (with immunolabeling) for cell components and compartments, and in Chapter 14, quantitative fluorescence, including issues associated with fluorescence intensity measurements and membrane potential of the dyes. In the fifteenth chapter, Cox introduces the reader to advanced fluorescence techniques, including fluorescence lifetime imaging (FLIM)) and fluorescence resonant energy transfer (FRET). In the final chapter (Chapter 16), he summarizes the developments in optical microscopy and then takes a closer look at scanning probe microscopy and atomic force microscopy, to name a few.
I like that Cox added four appendices, in which he discusses the important issue of microscope care and maintenance (Appendix 1), ways of keeping cells alive under the microscope (Appendix 2), antibody labeling of plant and animal cells (Appendix 3), and image processing with ImageJ, an image processing software program (Appendix 4). The book also contains a functional 10-pages index, but lacks a glossary, which I believe would have been helpful to the reader. Nevertheless, this is a fascinating book, a “must-have” for any microscopist working with the basic unit of life: the cell. Furthermore, I believe that microscopy facilities as well as life science libraries should carry a copy of this important book.
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